OAD
Collection of tools and scripts useful to automate microscopy workflows in ZEN Blue using Python and Open Application Development tools.
Open Application Development (OAD)
ZEN Blue is an open, flexible and powerful image acquisition platform that allows controlling a wide range of microscopes systems. Additionally it offers various tools to automate microscopy workflows including acquisition, image analysis and image processing tasks.
In order to fulfill the request for automation the ZEN Blue platform offers various features and options, which are combined inside a concept called Open Application Development (OAD).
Its main components are:
- CZI image data format and its APIs
- Python Scripting (OAD Simple API) using IronPython
- ZEN API Control ZEN from the outside
- Interfaces to ZEN (TCP-IP, COM, Extensions)
- Experiment Feedback - Adaptive Acquisition with Online Image Analysis
OAD - General Concept and Key Features
Open Application Development (OAD) uses powerful Python Scripts to simplify, customize and automate your workflows.
Analyze and Exchange data with applications like Fiji, Python, Knime, CellProfiler, Icy, MATLAB, Excel and …
API for reading and writing CZI image data using custom software
- ZeissImgLib (.NET) to be used on Windows-based systems
- libCZI (C++) and pylibCZIrw (python) for cross-platform applications
- BioFormats (CZIReader) allow easy access to CZI files from many external applications using the BioFormats library
BioFormats Import as a module inside ZEN Blue as well as OME-TIFF Export
Create “smart” experiments with Experiment Feedback and modify the acquisition On-the-fly based on Online Image Analysis and External Inputs
Use "Guided Acquisition" and "Automated Photomanipulation" modules in ZEN
ZEN Interfaces
Automated Dynamics
External Software
Links and References
CZI Image Data Format for microscopes
libczi: Open Source Cross-Platform API to read and write CZI
pylibCZIrw: Open Source Cross-Platform API to read and write CZI from Python (based on libCZI C++) (Source Code)
Disclaimer
This is an collection of tools and scripts that is free to use for everybody. Carl Zeiss Microscopy GmbH's ZEN software undertakes no warranty concerning the use of those scripts, image analysis settings and ZEN experiments. Use them on your own risk.
Additionally Carl Zeiss Microscopy GmbH's ZEN software allows connection and usage to the third party software packages. Therefore Carl Zeiss Microscopy GmbH undertakes no warranty concerning those software packages, makes no representation that they will work on your system and/or hardware and will not be liable for any damages caused by the use of any scripts or examples inside this repository.
By using any of those examples you agree to this disclaimer.
Version: 2025.05.09
Copyright (c) 2025 Carl Zeiss AG, Germany. All Rights Reserved.